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Museum Shows

Cell Membranes Procedure

GOAL: Demonstrate to visitors that semipermeable membranes allow some materials to pass through but retain others.

MATERIALS:

Microscale: 3-L storage bottles containing:

Red watercolor (A)

Iron (III) chloride (FeCl 3), 0.1 M solution (B)

Potassium thiocyanate (KSCN), 0.02 M solution (B)

Potassium iodide (KI), 0.02 M solution containing iodine (C)

500-mL squeeze bottles for each of the solutions above

Soluble starch, 0.05% suspension (C) in 500-mL squeeze bottle

For each of 3 demonstrations (A, B and C):

Semipermeable membrane (2.5” circumference dialysis tubing), about 6” long, fastened at bottom

Beaker (150 mL)

Squeeze bottle and gallon jug of tap water

Paper towels

Waste bucket

1-2 extra 6” pieces of membrane, soaking in water

Macroscale: Rigid tube

Flexible tube

Objects of different sizes

PROCEDURE:

Set up:

1. Place 500-mL squeeze bottles with corresponding beaker containing membrane in order: Set A, Set B, Set C.

2. Have paper towels, squeeze bottle of water and waste bucket available.

During the Presentation:

1. Briefly discuss what a semipermeable membrane is, e.g., pore size, size of particles that pass through, use as cell membrane and in kidney dialysis.

2. Microscale: Explain that for the three tests (Set A, Set B and Set C), we will see by color changes whether particles went through in one direction, both directions or not at all.

a. Set A

• Water is outside the membrane (about 50 mL in beaker)
• Watercolor paint is inside the membrane (red color, membrane about 2/3 full)
• Tell visitors to look for color in the water if the paint can move through the membrane out into the beaker.
• Lower the membrane into the beaker and swirl. Lift out after about 10 seconds, then after 20 seconds of swirling to check for any color change in the beaker. (No color change.)

b. Set B

• FeCl 3 is outside the membrane (yellow, about 50 mL in beaker)
• KSCN is inside the membrane (colorless, membrane about 2/3 full)
• These two compounds can react to form an orange-brown complex.
• Ask for predictions if color change will occur inside the membrane, outside the membrane, neither or both.
• Lower membrane into beaker, swirl. Lift out after about 10 seconds, then after 20 seconds or swirling. (Both solutions should turn orange-brown to red-brown.)
• Confirm that visitors understand that this means particles were moving in both directions.

c. Set C

• Starch is outside the membrane (colorless, about 50 mL in beaker)
• Potassium iodide is inside the membrane (yellow, membrane about 2/3 full)
• The two solutions react to form a blue color.
• Ask for predictions if color change will occur inside the membrane, outside the membrane, neither or both.
• Lower membrane into beaker, swirl. Lift out after about 10 seconds, then after 20-30 seconds until blue color appears in beaker only.
• Confirm that visitors understand what this means.

3. Macroscale: Use the models provided.

a. Passive transport- Have visitors determine which objects go through the rigid tube.

b. Active transport- Have visitors determine which objects go through the flexible tube with squeezing (supplying energy), comparable to channels in the cell membrane.

Clean up:

1. Between each presentation, empty membranes and beakers and rinse to remove color.

2. When there is a break between presentations, soak the membranes in water. Leave in water overnight.

3. Contents of the waste bucket may be put down the drain. Make sure all color is thoroughly rinsed off the sink or toilet bowl.

EXPLANATION:

Cells in the body are surrounded by membranes that permit or deny access in and out of the cell to particles such as ions and molecules. The cell membranes allow active transport by supplying energy to permit entrance or exit.

The kidneys act as filters to remove toxic substances and other waste or excess materials from the blood. When kidneys fail, kidney dialysis can perform this function using a semipermeable membrane (see hand-out).

WHAT COULD GO WRONG?

1. In filling the membrane, if any solution spills on the outside, it must be rinsed off to prevent sudden or unexpected color change.

2. The soluble starch suspension in Set C needs to be aerated by shaking the 0.05% solution well each day to produce the blue color in a timely fashion. In addition, make up the diluted (0.05%) solution from the 0.5% stock solution every 5-7 days or as needed to produce a blue color within 15 seconds.

3. Speed of color change is based on the relative concentration of the two solutions and the total surface of membrane. Changing the membrane diameter, beaker size, or volume of solutions may speed up or slow down the color change.

GENERAL MAINTENANCE:

1. All solutions except starch may be prepared in bulk in 3-L containers and are stable for at least 3 months. Starch should be prepared as the 0.5% solution every 2 months and then diluted to 0.05% every 5-7 days.
2. Each presentation requires approximately 20 mL for solutions inside the membrane (watercolor, KSCN and KI), for a total of 150 presentations for each 3 L.

3. Each presentation requires approximately 50 mL for solutions in the beaker outside the membrane. For FeCl 3, 3 L is adequate for 60 presentations. For starch, each 300 mL of 0.5% stock solution is adequate for a total of 60 presentations (after portions are diluted to 0.05%).

4. Preparation of solutions:

5. Membrane maintenance

a. Prior to initial use, cut a piece of semipermeable membrane approximately 6” long, soak it in water for a few minutes and open it by gently sliding it back and forth between your fingers. (Do not allow to dry out as it becomes brittle and may tear.)

b. With gentle handling, the membrane should last for weeks. When it cracks or tears, discard it. Keep two new soaked and opened pieces of membrane on hand during presentations (see step a).

c. If the membrane becomes discolored (common with Set B), place it in about 30 mL of EDTA solution and heat in the microwave or on a hot plate. Microwave for 30-60 seconds (or bring to a boil). The membrane should change from yellow-brown to colorless or very faint yellow. Rinse the membrane and store in water.